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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

AccuPower® RocketScript™ RT PreMix


  • Product from BIONEER

  • Ready to use RT Mastermix - just add template and primers and start reverse transcription

  • Efficient cDNA synthesis up to 10 kb

  • Excellent performance on complex templates due to high thermostability of RocketScript RT (42°C~70°C)

  • Lyophilized mastermix is stable at room temperature for a month and for 2 years at -20°C

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Description

AccuPower® RocketScript™ RT PreMix contains BIoneer’s exclusive M-MLV based thermostable reverse transcriptase, RocketScript™.

RocketScript™ Reverse transcriptase is much more thermostable compared to native M-MLV.  RocketScript™ is active at 42°C~70°C, allowing efficient cDNA synthesis from virtually any RNA, including complex-structured ones. The lyophilized PreMix contains all components necessary for a successful reverse transcription reaction, including RTase, RNase inhibitor and buffer components. Just add template RNA, primers and water, and the RT reaction is ready to go.


Amplification

Specifications

Specifications

  • 5' to 3' exonuclease: No
  • 3' to 5' exonuclease: No
  • 3’ – A Overhang: No
  • Fragment Size: Up to 10 kb

Applications

  • First-strand synthesis of cDNA from RNA molecules (RT)
  • RT-PCR
  • Random priming reaction
  • Library construction
  • Probe labeling
  • mRNA 5end Mapping by Primer Extension Analysis
  • Real time PCR

Supporting Data

References

Resources

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