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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

AccuPower® Rocketplex RT-PCR PreMix


  • Product from BIONEER

  • Ready to use RT-PCR mastermix for one step RT-PCR with multiple primer pairs

  • Thermostable RocketScript™ RT active at high temperatures (up to 70°C)

  • Enzyme-mediated hot start technology ensures highest sensitivity and specificity

  • Amplification of 1 - >10 target genes in one tube

  • Premixed with electrophoresis dye for direct gel loading

  • Lyophilized mastermix is stable at room temperature for a month and for 2 years at -20°C

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Description

AccuPower® Rocketplex RT-PCR PreMix is a ready mastermix for RT-PCR with multiple primer pairs. The mix includes proprietary exceptionally thermostable Reverse transcriptase RocketScript™ which is active at high temperatures (up to 70°C) and can perform efficient cDNA synthesis even on RNA templates of very complex secondary structure. Subsequent PCR employs bioneer enzyme -mediated hot start technology what ensures highest sensitivity and low background amplification, highly specific products are obtained even with multiple primers. AccuPower® Rocketplex RT-PCR PreMix allows amplification of 1 to more than 10 target genes in one tube.

AccuPower® Rocketplex RT-PCR PreMix contains all the components necessary for cDNA synthesis and PCR of multiple targets in one tube (one-step RT-PCR). The RT-PCR PreMix consists of both RocketScript™ Reverse Transcriptase, an RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer, RNase inhibitor, loading dye and stabilizer. The PCR reactions can be loded directly on the gel without adding a loading dye.


Amplification

Specifications

Specifications

  • 5' to 3' exonuclease: No
  • 3' to 5' exonuclease: No
  • 3' – A Overhang: Yes
  • Fragment Size: Up to 1 kb

Applications

  • Multiplex RT-PCR
  • Low copy detection
  • Gene-expression analysis

Supporting Data

References

Resources

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