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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

AccuPower® CycleScript RT PreMix


  • Product from BIONEER

  • Ready to use RT Mastermix with different primers - just add template and start reverse transcription

  • Cyclic RT technology allows for a fast and efficient RT reaction - synthesize up to 9 kb transcripts in just 10 minutes

  • Available as prealiquoted lyophilized mastermix

  • Lyophilized mastermix is stable at room temperature for a month and for 3 years at -20°C

Inquire prices at value@bts-biotech.com

Catalogue # Product Name Unit Size
K-2044 AccuPower® CycleScript RT PreMix dT20 / 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes 96 r of 20 µl
K-2044-B AccuPower® CycleScript RT PreMix dT20 / 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes 480 r of 20 µl
K-2047 AccuPower® CycleScript RT PreMix dT20 / 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes 96 r of 50 µl
K-2047-B AccuPower® CycleScript RT PreMix dT20 / 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes 480 r of 50 µl
K-2050 AccuPower® CycleScript RT PreMix dT20 / 0.5 ml thin-wall tubes with attached cap / 100 tubes 100 r of 50 µl
K-2045 AccuPower® CycleScript RT PreMix dN12 / 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes 96 r of 20 µl
K-2045-B AccuPower® CycleScript RT PreMix dN12 / 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes 480 r of 20 µl
K-2048 AccuPower® CycleScript RT PreMix dN12 / 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes 96 r of 50 µl
K-2048-B AccuPower® CycleScript RT PreMix dN12 / 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes 480 r of 50 µl
K-2050-1 AccuPower® CycleScript RT PreMix dN12 / 0.5 ml thin-wall tubes with attached cap / 100 tubes 100 r of 50 µl
K-2046 AccuPower® CycleScript RT PreMix dN6 / 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes 96 r of 20 µl
K-2046-B AccuPower® CycleScript RT PreMix dN6 / 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes 480 r of 20 µl
K-2049 AccuPower® CycleScript RT PreMix dN6 / 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes 96 r of 50 µl
K-2049-B AccuPower® CycleScript RT PreMix dN6 / 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes 480 r of 50 µl
K-2050-2 AccuPower® CycleScript RT PreMix dN6 / 0.5 ml thin-wall tubes with attached cap / 100 tubes 100 r of 50 µl

Inquire prices at value@bts-biotech.com

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Description

AccuPower® CycleScript RT (available with: dT20, dN12, or dN6 primers) PreMix is an easy to resuspend lyophilized PCR Master Mix including CycleScript Reverse Transcriptase, primer, and all other components for cDNA synthesis. The mix is lyophilized and conveniently prealiquoted in individual tubes. Simply add template RNA, and water. The reverse transcription is performed by either a Cyclic RT reaction (patent pending) or conventional reverse transcription PCR. The use of Cyclic RT reaction allows for better results compared to conventional reverse transcription PCR, especially for rare transcripts. The resulting cDNA can be used in a variety of applications such as reverse transcription PCR (RT-PCR). The AccuPower® CycleScript RT (dT20, dN12, or dN6) PreMix series is stable for 3 years at - 20°C due to a patented stabilizer.

AccuPower® CycleScript RT was developed for both a conventional reverse transcription PCR at a fixed temperature at 42°C as well as Cyclic Reverse Transcription that is carried out like a PCR (please see the following comparison table). Bioneer’s Cyclic RT is an innovative technology to synthesize more homogeneous cDNA in less time compared to the conventional reverse transcription.

Conventional Reverse Transcription

Step 1: RNA denaturation at 65°C for 10 minutes
Step 2: cDNA synthesis at a temperature between 37°C ~ 55°C for 15 ~ 60 minutes

Cyclic Reverse Transcription

Step 1: Primer annealing at a temperature between 15°C and 40°C for 30 seconds
Step 2: cDNA synthesis at a temperature between 42°C and 48°C for 4 minutes
Step 3 (optional): Denaturation of the secondary structure of the RNA template and cDNA synthesis at a temperature between 50°C and 55°C for 30 seconds

 


Amplification

Specifications

Specifications

  • 3' to 5' exonuclease: No
  • 3’ – A Overhang: No
  • Fragment size: 9 kb

Applications

  • cDNA synthesis

Supporting Data

References

Resources

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