Description
AccuPower® CycleScript RT (available with: dT20, dN12, or dN6 primers) PreMix is an easy to resuspend lyophilized PCR Master Mix including CycleScript Reverse Transcriptase, primer, and all other components for cDNA synthesis. The mix is lyophilized and conveniently prealiquoted in individual tubes. Simply add template RNA, and water. The reverse transcription is performed by either a Cyclic RT reaction (patent pending) or conventional reverse transcription PCR. The use of Cyclic RT reaction allows for better results compared to conventional reverse transcription PCR, especially for rare transcripts. The resulting cDNA can be used in a variety of applications such as reverse transcription PCR (RT-PCR). The AccuPower® CycleScript RT (dT20, dN12, or dN6) PreMix series is stable for 3 years at - 20°C due to a patented stabilizer.
AccuPower® CycleScript RT was developed for both a conventional reverse transcription PCR at a fixed temperature at 42°C as well as Cyclic Reverse Transcription that is carried out like a PCR (please see the following comparison table). Bioneer’s Cyclic RT is an innovative technology to synthesize more homogeneous cDNA in less time compared to the conventional reverse transcription.
Conventional Reverse Transcription
| Step 1: | RNA denaturation at 65°C for 10 minutes |
| Step 2: | cDNA synthesis at a temperature between 37°C ~ 55°C for 15 ~ 60 minutes |
Cyclic Reverse Transcription
| Step 1: | Primer annealing at a temperature between 15°C and 40°C for 30 seconds |
| Step 2: | cDNA synthesis at a temperature between 42°C and 48°C for 4 minutes |
| Step 3 (optional): | Denaturation of the secondary structure of the RNA template and cDNA synthesis at a temperature between 50°C and 55°C for 30 seconds |
Specifications
Specifications
- 3' to 5' exonuclease: No
- 3’ – A Overhang: No
- Fragment size: 9 kb
Applications
- cDNA synthesis
Supporting Data
References
Resources
Manual
• AccuPower® CycleScript RT PreMix (dN6)
• AccuPower® CycleScript RT PreMix (dN12)
• AccuPower® CycleScript RT PreMix (dN20)
MSDS
• MSDS_AccuPower® PCR Master Mixes
Brochure
• AccuPower® PCR Master Mixes - 2010 Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
