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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

AccuPrep® Stool DNA Extraction Kit


  • Product from BIONEER

  • Column-based gDNA extraction kit for high yield of gDNA from various stool samples

  • Optimized lysis buffer ensures efficient lysis of different origin samples and eliminated PCR inhibitors

  • High column binding capacity, excellent purity DNA for all demanding applications

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Description

AccuPrep® Stool DNA Extraction Kit is designed for the rapid, convenient extraction of DNA from fresh or frozen stool or other samples containing large amounts of material that can inhibit PCR. This is a conventional silica column based kit eliminating the use of organic solvents. Using the specially optimized lysis buffer, the efficient lysis of diffrent samples is achieved and spin-column finally eliminates contaminants and enzyme inhibitors (such as heparin, bilirubin bile salts, porphyrin)  finally the high-purity DNA is obtained, ready for use in a variety of applications. Proteinase K powder, supplied in the kit, can additionally be ordered separately if required.

Principle

principle

Components

kit

Typical Yield

 yields
Fig. 1 Gel electrophoresis of stool genomic DNA extracted by AccuPrep® Stool DNA Extraction Kit
Lane M1: Molecular weight marker, λ DNA/EcoR l + Hind lll Markers (D-1070, Bioneer),
Lane M2: Molecular weight marker, 1 kb ladder (D-1040, Bioneer),
Lane 1~3: DNA purified with Bioneer's AccuPrep Stool DNA Extraction Kit.
100ng of Intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)

Specifications

Specifications

Sample Amount Yield
Stool 100 mg 2 ~ 5 ug

Content

Components K-3036 (100 reaction)
Stool Lysis Buffer (SL) 50 ml X 1 ea
Binding Buffer (ST) 50 ml X 1 ea
Washing Buffer 1 (W1) 40 ml X 1 ea
Washing buffer 2 (W2) 20 ml X 1 ea
Elution Buffer (E) 25 ml X 1 ea
Proteinase K, lyophilized 25 mg X 2 ea
DNA binding column tube 100 ea X 1 pack
2.0ml tubes for filtration 100 ea X 1 pack
1.5ml tubes for elution 100 ea X 1 pack
Manual 1 ea

Supporting Data

References

Resources

Manual

• Manual_AccuPrep® Stool DNA Extraction Kit

Brochure

• DNA-RNA Preparation 2010 Brochure

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

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