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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

ExiPrep™ 16 Plus System and Accessories


  • Product from BIONEER

  • Easy-to-use instrument for fast and efficient automated NA or protein purification

  • Walk-away automation, contamination shield and UV sterilization - for maximum efficiency and no cross-contamination

  • Combined heating/magnetic block facilitates elution and allows for unsurpassed yields


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Description

ExiPrep™ 16 Plus is a laboratory automation system for extraction of DNA or RNA from various biological samples including tissue, cell culture, whole blood, serum, plasma, swab, bacteria, yeast, etc. It features the ability to purify His-tag recombinant proteins as well.

ExiPrep™ 16 Plus can handle up to 16 samples at once and includes optimized extraction protocols for many sample types what allows for the unsurpassed results in nucleic acid or protein purification. The ExiPrep™ 16 Plus has advanced features such as an intuitive touch screen, a UV sterilization cycle and a unique contamination shield, each designed to provide an extra level of confidence in excellent results.

ExiPrep

   Preinstalled Optimized Protocols for Maximum Results
newsfigure4 ExiPrep™ 16 Plus contains over 900 optimized protocols for nucleic acid extraction and protein purification. Each protocol is fine-tuned to sample and target type to give anybody reproducible results every experiment.
The instrument software can also be upgraded through the network connection port so you can stay up-to-date with the best performing protocols.

 

  Easy-to-use Touchscreen interface
newsfigure3 The responsive 3.5 inch touchscreen enables simple instrument operation. From selecting sample-optimized protocols to turning the UV sterilization lamp on and off, the touchscreen is designed for optimal workflow. During instrument operation, the touchscreen will give real-time feedback on the progress of the extraction, maximizing user convenience.

 

  Automatic UV Sterilization Lamp
newsfigure5 The ExiPrep™ 16 Plus has a powerful UV sterilization lamp that enables the user to sterilize the instrument chamber before and/or after every nucleic acid extraction or protein purification run. This prevents possible inter-assay cross-contamination that may occur on a busy work day.

 

  Contamination Shield
newsfigure6 ExiPrep™ 16 Plus comes with a contamination shield designed to protect the assay from cross-contamination during instrument operation. Any time the pipette tips are moving, the contamination shield will slide under the tips, therefore eliminating the possibility of intra-assay cross-contamination which is critical when working with multiple samples.

 

  Combined Magnetic/Heating Block
newsfigure5 To increase nucleic acid extraction and protein purification efficiency, the ExiPrep™ 16 Plus has an integrated combined magnetic/heating block. The combination of bead magnetization and sample heating reduces experiment time and increases elution efficiency, resulting in increased sample yield. (Patent pending)Process: Read clockwise.



Procedure

Specifications

Applications

Site Application
University and Corporate Laboratories Nucleic acid extraction and protein purification from various samples
University Hospital research center laboratories Nucleic acid extraction for research development from blood, tissue and various biological samples
Food companies Nucleic acid extraction for GMP/Food poisoning test from various food ingradients
Military, Police Nucleic acid extraction for the detection of biological terror bacteria or Food poisoning test

Specifications

   Dimensions (W X H X D) 320 mm x 487 mm x 535 mm
   Weight 22 kg
   Temperature range 15 - 30°C
   Humidity range 20 - 80%, no condensation
   Operating system Standalone
   User interface 320 x 240 touch screen graphic LCD
   Input Voltage 100 - 240 VAC
   Frequency 50 / 60 Hz
   UV sterilization 15 minute cycle
   Communications TCP/IP
   Heat block 40 - 90°C

Comparisons

  ExiPrep™ 16 Plus ExiProgen
DNA/RNA Extraction O O
Protein Synthesis X O
Protein Purification O
TFT Touch Screen O O
Sample 16 samples 16 samples
UV sterilization O O
Heater & magnetic block O O
Contamination shield of catridge O O
TCP/IP network X Multiple systems can be daisy-chained together (up to 6)
Cooling system for sample store X O
Automatic aliquoting X O
Power supply 24VDC, 2.7A(60W) 24VDC, 7.5A(180W)
weigh 22kg 27kg

* ExiPrep™ 16 Plus does not offer storing function. When you purify proteins using ExiPrep™ 16 Plus, you can use ExiPrep™ 16 Plus in the cold chamber or cold room.

Experimental Data

1. Genomic DNA from cultured mammalian cell (HeLa)
 Sample volume: 1X106 cells
 Protocol number: 109
 Total prep time: about 1hr 30min.
 Average yield: 4-8ug

 

Procedure

 

Agarose gel analysis


Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of cultured HeLa cell and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction.
Note all samples have very similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).



2. Genomic DNA from Bacteria (E.coli)
 Sample volume: 1X109 cells
 Protocol number: 109
 Total prep time: about 1hr 40min.
 Average yield: 8-12ug

 

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X109 cells of E.coli cells and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction.
Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).



3. Genomic DNA from Tissue (Bovine skeletal muscle)
 Sample volume: ~25mg
 Protocol number: 102
 Total prep time: about 1hr 30min.
 Average yield: up to 20ug

 

Procedure

 

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 25mg of bovine tissues and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction.
Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).



4. Genomic DNA from Plant Tissue (Spinach leaf)
 Sample volume: ~100mg
 Protocol number: 104
 Total prep time: about 1 hr
 Average yield: up to 5ug

 

Procedure

 

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 100mg of spinach leaves and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction.
Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).



5. RNA extraction from cultured mammalian cells (HeLa).
 Sample volume: 1.0 X 106 cells
 Protocol number: 202
 Average yield: up to 10 ug
 Average RQS: 9.5

 

Procedure

 

Electrophoresis result with LabChip GX

Results of capillary electrophoresis of total RNA extracted from HeLa cell(1X106 cells) using LabChip GX(Caliper life science, USA). Average RQS(RNA Quality Score) were at least 9.5t shown).

 

Real-time RT-PCR results of total RNA extracted from cultured cell

Real-time RT-PCR results using an AccuPower® RocketScrpt™ RT PreMix(K-2101, Bioneer) and AccuPower® Dualstar™ qPCR PreMix(K-6110, Bioneer) and run on Exicycler™ 96 Real-Time PCR System (A-2060, Bioneer) from total RNA extracted from HeLa cell(1X106 cells)

Supporting Data

References

Resources

Manual

ExiPrep™ 16 Plus

Brochure

ExiPrep™ 16 Plus

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

• CE - certificate

Technical Support

exiprep-support@bioneer.co.kr

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