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AccuPrep® Plasmid Mini Extraction Kit


  • Product from BIONEER

  • Conventional plasmid miniprep kit for high plasmid yields from variable bacterial culture volumes (1-10 ml)

  • Designed to be used for high to middle copy number plasmid DNA, for endA+ strains

  • High column binding capacity, excellent purity plasmid for all demanding applications

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Description

The AccuPrep® Plasmid Extraction Kit is designed for extraction of high-purity plasmid DNA from bacterial cultures such as E.coli. This is a conventional silica column based kit employing the modified alkaline lysis method. The extraction is carried out in three simple steps of binding/ washing/ elution, without the use of organic solvents. The kit works with different bacterial culture volumes from 1 to 10 ml. It also includes Endonuclease A denaturation buffer for the endA+ strains. For the downstream convenience,  50 well tube rack for the storage of the extracted plasmid DNA is supplied in the kit. RNase A powder, supplied in the kit, can additionally be ordered separately if required.

Principle

principle

Components

kit






Specifications

Specifications

   Starting culture volume 1 ml ~ 10 ml
   Column binding capacity > 20 ug
   Elution volume 50 – 100 ul
   Expected DNA yield Upto 20 ug
   Preparation time < 10 min

Content

Components K-3030 (200 reaction) K-3030-1 (50 reaction)
Buffer ① (Resuspension buffer) 60 ml X 1 ea 15 ml X 1 ea
Buffer ② (Lysis buffer) 60 ml X 1 ea 15 ml X 1 ea
Buffer ③ (Neutralization buffer) 80 ml X 1 ea 20 ml X 1 ea
Buffer ④ (Washing buffer) 16 ml X 2 ea 4 ml X 2 ea
Buffer ⑤ (Elution buffer) 24 ml X 1 ea 6 ml X 1 ea
Buffer D (Denaturation buffer) 75 ml X 1 ea 18 ml X 1 ea
RNase A, lyophilized 6 mg X 1 ea 1.5 mg X 1 ea
DNA binding column tube 50 ea X 4 box 50 ea X 1 box
Manual 1 ea 1 ea
One Page Protocol 1 ea 1 ea

 

Supporting Data

References

Resources

Manual

• Manual_AccuPrep® Plasmid Mini Extraction Kit

Brochure

• DNA-RNA Preparation 2010 Brochure

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

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