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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

ExiPrep® Plus Plant RNA Kit


  • Product from BIONEER

  • Reproducible results of automated extraction of RNA from plants

  • Excellent price-value ratio

  • High purity and high yield RNA with minimum hands-on time

Inquire prices at value@bts-biotech.com

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Description

ExiPrep® Plus Plant RNA Kit uses Plant lysis buffer to efficiently disrupt plant cell wall and proteins for efficient extraction of total RNA without degradation. The binding buffer and silica magnetic particles will bind total RNA to the surface of the beads. The reaction containers are then subject to a magnetic field, forcing the total RNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers +are introduced sequentially during in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the total RNA-bound silica magnetic particles remain bound by the magnetic field. RNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified total RNA.

The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance. The buffer composition was developed to extract high purity and high yield NA suitable for the use in all  further applications.The extraction protocols programmed on the instrument were optimized for maximum performance. Everybody can extract NA of high purity and yield using Bioneer instruments and automated kits

Procedure




Experimental Data

Fig. 1
Fig. 1 Electrophoresis result with extracted plant total RNA
Results of gel electrophoresis of total RNA extracted from various plant samples. Expected yields were on average 5-15ug depending on sample types. Also expected purities were at least 2.0 (A260/A280) and 1.5 (A260/A230).

 

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Specifications

Specifications

  Leaf tissue (wet) Seed
  Chinese cabbage Broccoli Chinese cabbage Red bean
Sample Vol. 100 mg 100 mg 100 mg 100 mg
Elution Vol. 50 ul 50 ul 50 ul 50 ul
Expected yield (ug) Up to 15 ug Up to 8 ug Up to 5 ug Up to 9 ug
Purity (A260/280) > 2.0 > 2.0 > 2.0 > 2.0
Purity (A260/280) > 2.0 > 2.1 > 1.8 > 1.5

Content

Components Quantity Note
Buffer Cartridge ① 6 ea  
Buffer Cartridge ② 6 ea  
Plant Lysis buffer ① 1 ea 25 ml
Plant Lysis buffer ② 1 ea 25 ml
Disposable filter tip 3 pack 32 ea/pack
Elution tube 1 pack 12 ea/pack
Manual 1 ea  

Supporting Data

References

Resources

Manual

ExiPrep™ 16 Plus

Brochure

ExiPrep™ 16 Plus

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

• CE - certificate

Technical Support

exiprep-support@bioneer.co.kr

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