Description
ExiPrep® Plus Rice Genomic DNA Kit is designed for automated DNA extraction from rice seeds using ExiPrep® instrument
Rice Genomic DNA Kit uses a unique lysis buffer with Proteinase K to efficiently dissrupt tissues and proteins for efficient extraction of genomic DNA.The binding buffer and silica magnetic particles will bind DNA to the surface of the beads. The wells are then subject to a magnetic field, forcing the DNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the DNA-bound silica magnetic particles remain bound by the magnetic field. DNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified DNA.
The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance. The buffer composition was developed to extract high purity and high yield NA suitable for the use in all further applications.The extraction protocols programmed on the instrument were optimized for maximum performance. Everybody can extract NA of high purity and yield using Bioneer instruments and automated kits.
Procedure
Experimental Data
Fig. 1 Agarose gel electrophoresis results of genomic DNA extracted from rice sample
Results of gel electrophoresis of genomic DNA extracted from rice sample. Yields were on average 1-2ug and purities (A260/A280) were at least (average) 1.8.
Fig. 2 Multiplex conventional PCR results of genomic DNA extracted from rice sample
Results of gel electrophoresis of PCR product using its specific PCR primer sets to distinguish its cultivar varieties.
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Specifications
Specifications
| Starting culture volume | up to 20 mg |
| Elution volume | 50 ul |
| Expected Yield | 1 - 2 ug |
| Expected Purity (A260/280) | > 1.8 |
Content
| Components | Quantity | Note |
| Buffer Cartridge ① | 6 ea | |
| Buffer Cartridge ② | 6 ea | |
| Rice Lysis buffer | 1 ea | 40 ml |
| Rice Binding buffer | 1 ea | 20 ml |
| Proteinase K, lyophilized | 2 tube | 20 mg/ tube |
| Disposable filter tip | 3 pack | 32 ea/pack |
| Elution tube | 1 pack | 12 ea/pack |
| Manual | 1 ea |
Supporting Data
References
Resources
Manual
Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• EN ISO 13485:2003 / AC:2009 - certificate
