Description
ExiPrep® Plus Blood Genomic DNA Kit is designed for automated DNA extraction from blood using ExiPrep® instrument.
Kit uses a unique lysis buffer with Proteinase K to lyse eliminate proteins for efficient extraction of DNA. The binding buffer and silica magnetic particles will bind DNA to the surface of the beads. The wells are then subject to a magnetic field, forcing the DNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the DNA-bound silica magnetic particles remain bound by the magnetic field. DNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified DNA.
The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance. The buffer composition was developed to extract high purity and high yield NA suitable for the use in all further applications.The extraction protocols programmed on the instrument were optimized for maximum performance. Everybody can extract NA of high purity and yield using Bioneer instruments and automated kits.
Procedure
Experimental Data
Fig. 1 Agarose gel electrophoresis results of genomic DNA extracted from mouse tissue
Results of gel electrophoresis of genomic DNA extracted from 25mg mouse lung tissue. Yields were on average 15-20ug and purities (A260/A280) were at least (average) 1.8. Also, to observe cross-contamination that may occur during the extraction process, the mouse tissue samples were placed in a checkerboard pattern, and the rest of the wells were filled with D.W. No cross-contamination could be detected. M; Size marker, S; Extraction with mouse tissue sample, B; Extraction with D.W only.
Specifications
Specifications
| Starting culture volume | up to 200 ul |
| Elution volume | 50 - 100 ul |
| Expected Yield | 5 - 20 ug |
| Expected Purity (A260/280) | > 1.8 |
Content
| Components | Quantity | Note |
| Buffer Cartridge ① | 6 ea | |
| Buffer Cartridge ② | 6 ea | |
| Tissue lysis buffer | 1 ea | 25 ml |
| Disposable filter tip | 3 pack | 32 ea/pack |
| Elution tube | 1 pack | 12 ea/pack |
| Manual | 1 ea |
Supporting Data
References
Resources
Manual
Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• EN ISO 13485:2003 / AC:2009 - certificate
