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Description

ProFi Taq DNA polymerase, developed by Bioneer, is a unique recombinant Taq DNA polymerase that offers enhanced amplification efficiency for PCR. ProFi Taq DNA polymerase provides more efficient amplification and higher fidelity than conventional Taq DNA polymerase. This enzyme is applicable to any template DNA, and especially effective in amplifying large genomic DNA fragments up to 20 kb. ProFi Taq DNA polymerase provides accurate long-range amplification of standard and complex templates and amplification of low-copy target, and is highly suitable for all PCR applications.

For maximum flexibility, enzyme is available with or without dNTPs, with buffer containing magnesium or with magnesium supplied separately.

Specifications

Specifications

  • 5' to 3' exonuclease activity: Yes
  • 3' to 5' exonuclease activity: No
  • 3’ – A Overhang: Yes
  • PCR product size: ~ 30 kb

Applications

  • Primer extension
  • Long-range amplification from genomic DNA
  • GC rich PCR
  • Amplification of low-copy targets
  • High yield and high sensitivity PCR

Content

  • 10 x Reaction Buffer without MgCl2, Tris-HCl, KCl, pH 9.0
  • 10 x Reaction Buffer with MgCl2 Tris-HCl, KCl, 15 mM MgCl2, pH 9.0
  • Dilution Buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl,stabilizers, 50 % glycerol, pH 8.0
  • dNTP Mixture: 10 mM (2.5mM each dNTP)
  • MgCl2 Solution 20 mM

Supporting Data

References

Resources

Manual

ProFi Taq DNA polymerase

MSDS

• MSDS_ProFi Taq DNA polymerase

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

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