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Pfu DNA Polymerase


  • Product from BIONEER

  • Excellent enzyme for all high fidelity PCR applications

  • Low error rate amplification of up to 20 kb templates

  • Extremely temperature stable enzyme, providing high PCR yields

  • Attractive pricing for packages with dNTPs

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Description

Pfu DNA polymerase is a thermostable DNA polymerase isolated from Pyrococcus furiosus Vc1. It catalyzes the DNA-dependent polymerization of nucleotides into duplex DNA in the 5’ → 3’ direction and exhibits 3’ → 5’ exonuclease (proof reading) activity.  Pfu DNA polymerase is the ideal choice for a variety of techniques requiring high-fidelity DNA synthesis by PCR reaction.  It can apply to cloning, gene expression, site-directed mutagenesis and etc.

Bioneer Pfu retaining 94-99% of its thermostable activity after 1 hour at 95°C. Pfu DNA Polymerase is devoid of terminal transferase activity and generates blunt-ended PCR products.

Specifications

Enzyme Properties

  • 5' to 3' exonuclease activity: No
  • 3' to 5' exonuclease activity: Yes
  • 3’ – A Overhang: No
  • Fragment Size: < 20 kb

Applications

  • High Fidelity PCR
  • Gene synthesis
  • Blunt-end PCR Cloning
  • Mutagenesis

Reagents Supplied

  • 10 x Reaction Buffer: MgSO4, Tris-HCl, KCl, (NH4)2SO4, Acetylated BSA pH 8.8
  • 1 x Dilution Buffer with 50% glycerol.
  • dNTP Mix: 2.5 mM of each dNTP

Concentration

2.5 U/μl

Store 

-20°C

Unit Definition

One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.

Supporting Data

References

Resources

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