Description
Pfu DNA polymerase is a thermostable DNA polymerase isolated from Pyrococcus furiosus Vc1. It catalyzes the DNA-dependent polymerization of nucleotides into duplex DNA in the 5’ → 3’ direction and exhibits 3’ → 5’ exonuclease (proof reading) activity. Pfu DNA polymerase is the ideal choice for a variety of techniques requiring high-fidelity DNA synthesis by PCR reaction. It can apply to cloning, gene expression, site-directed mutagenesis and etc.
Bioneer Pfu retaining 94-99% of its thermostable activity after 1 hour at 95°C. Pfu DNA Polymerase is devoid of terminal transferase activity and generates blunt-ended PCR products.
Specifications
Enzyme Properties
- 5' to 3' exonuclease activity: No
- 3' to 5' exonuclease activity: Yes
- 3’ – A Overhang: No
- Fragment Size: < 20 kb
Applications
- High Fidelity PCR
- Gene synthesis
- Blunt-end PCR Cloning
- Mutagenesis
Reagents Supplied
- 10 x Reaction Buffer: MgSO4, Tris-HCl, KCl, (NH4)2SO4, Acetylated BSA pH 8.8
- 1 x Dilution Buffer with 50% glycerol.
- dNTP Mix: 2.5 mM of each dNTP
Concentration
2.5 U/μl
Store
-20°C
Unit Definition
One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.
Supporting Data
Brochure
References
Resources
Manual
MSDS
Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
