Description
Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase. This enzyme is able to use an RNA molecule as a template and synthesize a single-stranded DNA. M-MLV Reverse Transcriptase is isolated from a recombinant E.coli strain containing a plasmid with M-MLV gene.
This is a classical enzyme ideal for standard cDNA synthesis reactions. Bioneer suppliers it in economical packages with dNTPs and ensures enzyme being free from DNases and RNases what makes your template and synthesized cDNA safe.
Specifications
Enzyme Properties
- 5' to 3' exonuclease activity: No
- 3' to 5' exonuclease activity: No
- 3’ – A Overhang: No
- Strand Displacement: Yes Fragment Size: Up to 9 kb
Application
First strand cDNA synthesis from RNA, RT-PCR, and qRT-PCR
Reagents Supplied
- 5 x Reaction Buffer: 50 mM Tris-HCl, 250 mM KCl, 10 mM MgCl2, pH 8.1
- 100 mM DTT
- dNTP Mix: 2.5 mM of each dNTP
Concentration
200 U/μl
Storage Conditions
50% glycerol containing 20 mM Tris-Cl (pH7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % IGEPAL CA-630
Store
-20°C
Unit Definition
One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly (A)oligo (dT) as template primer.
Supporting Data
References
Resources
Manual
MSDS
• MSDS_M-MLV Rtase dilution buffer
Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
