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MMLV Reverse Transcriptase


  • Product from BIONEER

  • Classical enzyme for cDNA synthesis, free from DNases and RNases

  • Economical package - in addition to the buffer, includes dNTPs

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Description

Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase. This enzyme is able to use an RNA molecule as a template and synthesize a single-stranded DNA. M-MLV Reverse Transcriptase is isolated from a recombinant  E.coli strain containing a plasmid with M-MLV gene.

This is a classical enzyme ideal for standard cDNA synthesis reactions. Bioneer suppliers it in economical packages with dNTPs and ensures enzyme being free from DNases and RNases what makes your template and synthesized cDNA safe.

Specifications

Enzyme Properties

  • 5' to 3' exonuclease activity: No
  • 3' to 5' exonuclease activity: No
  • 3’ – A Overhang: No
  • Strand Displacement: Yes Fragment Size: Up to 9 kb

Application

First strand cDNA synthesis from RNA, RT-PCR, and qRT-PCR

Reagents Supplied

  • 5 x Reaction Buffer: 50 mM Tris-HCl, 250 mM KCl, 10 mM MgCl2, pH 8.1
  • 100 mM DTT
  • dNTP Mix: 2.5 mM of each dNTP

Concentration

200 U/μl

Storage Conditions

50% glycerol containing 20 mM Tris-Cl (pH7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % IGEPAL CA-630

Store 

-20°C

Unit Definition

One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly (A)oligo (dT) as template primer.

Supporting Data

References

Resources

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