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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

HotStart DNA Polymerase


  • Product from BIONEER

  • Unique enzyme-mediated HotStart results in greater specificity and more robust reactions

  • Top DNA Polymerase allows for better yields, as it is 3 times more processive than Taq

  • Excellent enzyme for all hot start PCR applications

  • Highly specific amplification of up to 12 kb targets

  • Attractive pricing for packages with dNTPs

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Description

Bioneer’s HotStart DNA Polymerase uses an exclusive enzyme-mediated HotStart PCR technology that supresses polymerization reaction at room temperature and completely releases all polymerase activity during the first denaturation step.
Top
DNA polymerase is completely inhibited by pyrophosphate at temperatures below 70°C. However, at temperatures above 70°C, a thermostable pyrophosphatase initiates pyrophosphate hydrolysis and activates the DNA polymerase. This prevents the formation of non-specific products and primer-dimers during the reaction set-up process and results in improved PCR specificity.

It is ideal for nucleic acid amplification reactions involving complex genomic or cDNA templates, very low copy targets, and multiplex reactions.

Specifications

Enzyme Properties

  • 5' to 3' exonuclease activity: No
  • 3' to 5' exonuclease activity: No
  • 3’ – A Overhang: Yes
  • Fragment Size: Up to 12 kb

Applications

  • HotStart PCR, PCR with complex genomic templates/low copy templates/cDNA
  • Multiplex PCR
  • Primer extension
  • SNP typing
  • Real-Time PCR using SYBR Green dye

Reagents Supplied

  • 10 x Reaction Buffer : Tris-HCl, KCl, Pyrophosphate, pH 9.0
  • 1 x Dilution Buffer : 50% glycerol containing 50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, pH 8.2
  • dNTP Mix, 10 mM : 2.5mM of each dNTP
  • 20 mM MgCl2

Concentration

5 U/μl

Storage Conditions

50% glycerol containing 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, pH 8.0

Store Temperature

-20°C

Unit Definition

One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.

Supporting Data

References

Resources

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