Description
Get more cDNA in less time with CycleScript Reverse Transcriptase. CycleScript is a versatile reverse transcriptase - applicable to both conventional Reverse Transcription and Cyclic Reverse Transcription (Cyclic RT, patent pending – for cDNA amplification). It features high activity across a wide range of temperatures from 37 to 55°C therefore, reverse transcription is carried out like PCR. The Cyclic RT reaction has following steps: 1st incubation at 15~40°C for primer annealing, heating up to 42~48°C for extension, and finally incubation at 50~55°C for denaturation of the secondary structure of the RNA (optional). Bioneer's novel Cyclic RT system offers homogeneous cDNA synthesis, with a high yield of cDNA up to 9 kb.
Specifications
Enzyme Properties
- 5' to 3' exonuclease activity: No
- 3' to 5' exonuclease activity: No
- 3’ – A Overhang: Yes
- Strand Displacement: Yes
- Fragment Size: Up to 9 kb
Applications
- First-strand synthesis of cDNA from RNA molecules
- RT-PCR
- Random priming reaction
- Library construction
- Probe labeling
- mRNA 5’ end mapping by primer extension analysis
Reagents Supplied
- 5 x Reaction Buffer: 50 mM Tris-HCl, 250 mM KCl, 10 mM MgCl2, pH 8.1
- 100 mM DTT
- dNTP Mix: 2.5 mM of each dNTP
Concentration
200 U/μl
Storage Conditions
50% glycerol containing 20 mM Tris-Cl (pH7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % IGEPAL CA-630
Store Temperature
-20°C
Unit Definition
One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly (A)ooligo (dT) as template primer.
Supporting Data
Brochure
References
Resources
Manual
• CycleScript Reverse Transcriptase
MSDS
• MSDS_Cyclescript Rtase-dilution buffer
• MSDS_Cyclescript Rtase-reaction buffer
Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
