Description
AccuPower® RocketScript™ Cycle RT PreMix contains Bioneer’s exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript™. RocketScript™ Reverse Transcriptase shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.
The mix is lyophilized and conveniently prealiquoted in individual tubes. Simply add template RNA, and water. The reverse transcription is performed by bionner's Cyclic RT reaction (patent pending). The use of Cyclic RT reaction allows for better results compared to conventional reverse transcription PCR, especially for rare transcripts. The resulting cDNA can be used in a variety of applications such as reverse transcription PCR (RT-PCR). T
Bioneer’s Cyclic RT is an innovative technology to synthesize more homogeneous cDNA in less time compared to the conventional reverse transcription.
Conventional Reverse Transcription
| Step 1: | RNA denaturation at 65°C for 10 minutes |
| Step 2: | cDNA synthesis at a temperature between 37°C ~ 55°C for 15 ~ 60 minutes |
Cyclic Reverse Transcription
| Step 1: | Primer annealing at a temperature between 15°C and 40°C for 30 seconds |
| Step 2: | cDNA synthesis at a temperature between 42°C and 48°C for 4 minutes |
| Step 3 (optional): | Denaturation of the secondary structure of the RNA template and cDNA synthesis at a temperature between 50°C and 55°C for 30 seconds |
Specifications
Specifications
- 5' to 3' exonuclease: No
- 3' to 5' exonuclease: No
- 3’ – A Overhang: No
- Fragment Size: Up to 10 kb
Applications
- First-strand synthesis of cDNA from RNA molecules (Reverse Transcription)
- RT-PCR
- Random priming reaction
- Library construction
- Probe labeling
- mRNA 5' end mapping by primer extension analysis
Supporting Data
References
Resources
Manual
• AccuPower® RocketScript Cycle RT PreMix
MSDS
• MSDS_AccuPower® RocketScript Cycle RT PreMix
Brochure
• RocketScript Reverse Transcriptase - 2011 Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
