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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

AccuPower® RocketScript™ Cycle RT PreMix


  • Product from BIONEER

  • Ready to use RT Mastermix - just add template and primers and start reverse transcription

  • Efficient cDNA synthesis up to 10 kb full size transcripts

  • Excellent performance on complex templates due to high thermostability of RocketScript RT (42°C~70°C)

  • Cyclic RT technology allows for a fast and efficient RT reaction

  • Lyophilized mastermix is stable at room temperature for a month and for 2 years at -20°C

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Description

AccuPower® RocketScript™ Cycle RT PreMix contains Bioneer’s exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript™. RocketScript™ Reverse Transcriptase shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.

The mix is lyophilized and conveniently prealiquoted in individual tubes. Simply add template RNA, and water. The reverse transcription is performed by  bionner's Cyclic RT reaction (patent pending). The use of Cyclic RT reaction allows for better results compared to conventional reverse transcription PCR, especially for rare transcripts. The resulting cDNA can be used in a variety of applications such as reverse transcription PCR (RT-PCR). T

Bioneer’s Cyclic RT is an innovative technology to synthesize more homogeneous cDNA in less time compared to the conventional reverse transcription.


Amplification

Conventional Reverse Transcription

Step 1: RNA denaturation at 65°C for 10 minutes
Step 2: cDNA synthesis at a temperature between 37°C ~ 55°C for 15 ~ 60 minutes

Cyclic Reverse Transcription

Step 1: Primer annealing at a temperature between 15°C and 40°C for 30 seconds
Step 2: cDNA synthesis at a temperature between 42°C and 48°C for 4 minutes
Step 3 (optional): Denaturation of the secondary structure of the RNA template and cDNA synthesis at a temperature between 50°C and 55°C for 30 seconds

 

 

Specifications

Specifications

  • 5' to 3' exonuclease: No
  • 3' to 5' exonuclease: No
  • 3’ – A Overhang: No
  • Fragment Size: Up to 10 kb

Applications

  • First-strand synthesis of cDNA from RNA molecules (Reverse Transcription)
  • RT-PCR
  • Random priming reaction
  • Library construction
  • Probe labeling
  • mRNA 5' end mapping by primer extension analysis

Supporting Data

References

Resources

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