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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

GeBAflex-tube Dialysis & Buffer Exchange Mini Kits (vol. 10-250 µl; MWCO: 6000-8000, 12000-14000; 25000)


  • Product from Gene Bio-Application Ltd (GeBA)

  • Easy procedure of protein or NA dialysis and buffer exchange

  • Protein, DNA, RNA extraction from all kind of gels

  • No special equipment or syringes required, amenable to high throughput

  • Unsurpassed sample recovery of >98%

  • A broad choice of volume sizes and of membrane MWCO cut-offs

  • Universal kits applicable for protein, peptide,  NA and oligo samples

  • Recovery of high purity samples excellent for demanding applications

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Description

The GeBAflex-tube Dialysis & Buffer Exchange Kits are for decade top-selling products for protein and nucleic acids dialysis, purification or buffer exchange applications.

GeBAflex-tube Dialysis & Buffer Exchange Kits include unique specially designed plastic single-use tube having an integrated permeable membrane enabling migration of salts. The high quality of the membrane allows for an unsurpassed 98% recovery of pure samples. The proprietary membrane is free from contaminating nucleic acids, heavy metals and treated with EDTA.

The kits perform in an easy-to-handle manner by pipetting the sample into the tube and performing dialysis while tubes are floating in the buffer. Even a floating rack is included for a convenience of use. Multiple samples at once can be processed what allows for high throughput applications. No special equipment or syringes are required.

The list of Applications of GeBAflex-tube Dialysis & Buffer Exchange Kits includes:

  • Protein dialysis or buffer exchange, sample concentration
  • Extraction of Proteins, RNA, DNA or oligos (>20 nt) from any gel matrix in any buffer
  • Removal of salts, stains or detergents from protein or NA samples
  • pH and buffer adjustment of protein or NA samples
  • Extraction of protein-protein, DNA-protein or RNA-protein complexes
  • Protein purification before automated protein sequencing or peptide mapping or MALDI-MS
  • Large-scale protein dialysis such as antibodies purification
  • Peptide dialysis, as small as 10 amino acids
  • Purification of Viral particles
  • Purification of tissue cell culture extracts

GeBAflex-tube Dialysis & Buffer Exchange Kits are available in different sizes adapted for differing sample volumes, they include appropriately sized tubes:

  • Mini Kits - Mini GeBAflex-tube, volume range 10 µl to 250 µl
  • Midi Kits - Midi GeBAflex-tube, volume range 50 µl to 800 µl
  • Maxi Kits - Maxi GeBAflex-tube, volume range 100 µl to 3000 µl (provided with changeable caps for flexible volume adjustments)
  • Mega Kits - Mega GeBaflex-tube, volume range 0,5 ml to 20 ml (changeable caps for volume adjustments can be purchased separately)

Gebaflex-tube

 

To cover broadest application range, eachsize GeBAflex-tube Dialysis & Buffer Exchange Kits are available with different membrane cut-offs defining membrane permeability for certain size molecules:

  1 kDa 3.5 kDa 6 – 8 kDa 12 – 14 kDa 25 kDa 50 kDa
Mini GeBAflex
(10 µl - 250 µl)
    6000-12000
MWCO
12000-14000
MWCO
25000
MWCO
 
Midi GeBAflex
(50 µl - 800 µl)
1000
MWCO
3500
MWCO
6000-12000
MWCO
     
Maxi GeBAflex
(100 µl - 3000 µl)
   3500
MWCO
6000-12000
MWCO 
12000-14000
MWCO
25000
MWCO
 50000
MWCO
Mega GeBaflex
(0,5 ml - 20 ml)
1000
MWCO
3500
MWCO
6000-12000
MWCO
12000-14000
MWCO
   


GeBAflex-tube is covered by the WO0190731 patent application assigned to Gene Bio-Application Ltd.

Specifications

Applications

  • Protein dialysis or buffer exchange, sample concentration
  • Extraction of Proteins, RNA, DNA or oligos (>20 nt) from any gel matrix in any buffer
  • Removal of salts, stains or detergents from protein or NA samples
  • pH and buffer adjustment of protein or NA samples
  • Extraction of protein-protein, DNA-protein or RNA-protein complexes
  • Protein purification before automated protein sequencing or peptide mapping or MALDI-MS
  • Large-scale protein dialysis such as antibodies purification
  • Peptide dialysis, as small as 10 amino acids
  • Purification of Viral particles
  • Purification of tissue cell culture extracts

GeBAflex-tube Dialysis & Buffer Exchange Kit sizes:

  • Mini Kits - Mini GeBAflex-tube, volume range 10 µl to 250 µl
  • Midi Kits - Midi GeBAflex-tube, volume range 50 µl to 800 µl
  • Maxi Kits - Maxi GeBAflex-tube, volume range 100 µl to 3000 µl (provided with changeable caps for flexible volume adjustments)
  • Mega Kits - Mega GeBaflex-tube, volume range 0,5 ml to 20 ml (changeable caps for volume adjustments can be purchased separately)

GeBAflex-tube Dialysis & Buffer Exchange Kit membrane cut-offs defining membrane permeability for certain size molecules:

  1 kDa 3.5 kDa 6 – 8 kDa 12 – 14 kDa 25 kDa 50 kDa
Mini GeBAflex
(10 µl - 250 µl)
    6000-12000
MWCO
12000-14000
MWCO
25000
MWCO
 
Midi GeBAflex
(50 µl - 800 µl)
1000
MWCO
3500
MWCO
6000-12000
MWCO
     
Maxi GeBAflex
(100 µl - 3000 µl)
   3500
MWCO
6000-12000
MWCO 
12000-14000
MWCO
25000
MWCO
 50000
MWCO
Mega GeBaflex
(0,5 ml - 20 ml)
1000
MWCO
3500
MWCO
6000-12000
MWCO
12000-14000
MWCO
   

Content

  • Each Kit contains appropriate size and given number of Single-use dialysis tubes and a floating rack.
  • Maxi kit contains also exchangeable tube caps for sample volume adjustment. For Mega tubes, the caps can be purchased separately.

Storage

Kits must be stored in a dry place at room temperature (15-25oC). Under these conditions, GeBAflex-tube kit can be stored for up to 12 months without any deterioration in performance and quality. For longer storage time, it is recommended that the GeBAflex-tube kit be stored in a cool place (refrigerator), at relative humidity of 35% at least.

Supporting Data

References

Resources

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