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Fast SeeBand Single Step Protein Staining Solution


  • Product from Gene Bio-Application Ltd (GeBA)

  • Fast single-step gel staining in 5-15 min

  • Non-toxic protein stain

  • No washing, no fixing of the gel before staining

  • No heating during staining, no destaining required

  • High sensitivity (10 ng) protein detection

  • No risk of overstaining, low background

  • Compatible with mass spectrometry

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Description

Fast SeeBand Single Step Protein Staining Solution is a non-toxic protein stain used for staining of polyacrylamide protein gels in a simple single step few minutes procedure. The staining procedure does not involve gel washing, fixation, boiling and destaining.

The solution is ready for use it provides fast results while leaving no overstaining concerns, as the gel background even during longer incubation is not colored. Only the proteins are stained and the background staining problems are completely eliminated what results in extremely well defined bands on highly transparent background. The gels stained with the Fast SeeBand Single Step Protein Staining Solution provide best defined detection without the background noice.

Fast SeeBand Single Step Protein Staining Solution provides the high staining sensitivity; protein bands having as low as 10 ng amounts can be detected. The stain formulation is non-toxic and does not contain methanol. Proteins stained with Fast SeeBand Single Step Protein Staining Solution are compatible with mass spectrometry analysis.

 High sensitivity of SeeBand Single Step Protein Staining Solution

2D gelsSeeBand Protein Staining

Specifications

Applications

Staining of polyacrylamide protein gels.

Proteins stained with Fast SeeBand Single Step Protein Staining Solution are compatible with mass spectrometry analysis.

Gel staining procedure for 8 x 10 cm mini gel 1.4 mm thick:

  • After electrophoresis, cut off the stacking gel.
  • Put the resolving gel in a container and add 25 ml of Fast SeeBand Single Step Protein Staining Solution
  • Shake the gel on an orbital shaker for 5-15 minutes. If the intensity is not enough, prolong the incubation to an hour or leave overnight.
  • Optionally: Wash the gel with water, especially in case of prolonged staining.

Storage

  • Store at 4oC. Keep the bottle firmly closed.
  •  Shipped at ambient temperature.
  •  This product is guaranteed for one year from date of purchase when properly handled and stored.

Supporting Data

References

GeBA homepage

 High sensitivity of SeeBand Single Step Protein Staining Solution

SeeBand Protein Staining

Resources

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