Description
ExiStation™ Viral DNA/RNA Kits are designed for automated viral DNA/RNA extraction from clinical samples using ExiStation™ system - a combined solution for Molecular Diagnostics including Automated NA extraction (ExiPrep™ 16 Dx) and qPCR (Exicycler™ 96)
Viral DNA/ RNA Kit is a product designed to extract high-purity viral DNA/ RNA from c serum, plasma, CSF, saliva, swab and cell free body fluid samples using the automated nucleic acid extraction instrument ExiPrep™ 16 Plus. The binding buffer and silica magnetic particles will bind viral DNA/ RNA to the surface of the beads. The reaction containers are then subject to a magnetic field, forcing the viral DNA/ RNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially during in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the viral DNA/ RNA-bound silica magnetic particles remain bound by the magnetic field. DNase RNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified viral DNA/ RNA.
The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance. The buffer composition was developed to extract high purity and high yield NA suitable for the use in all further applications.The extraction protocols programmed on the instrument were optimized for maximum performance. Everybody can extract NA of high purity and yield using Bioneer instruments and automated kits.
Procedure
Principle
Experimental Data
Fig. 1 Real-time PCR result(HBV) of viral DNA extracted from serum
Real-time PCR results using an AccuPower® HBV Quantitative PCR Kit(HBV-1111, Bioneer) and run on Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060, Bioneer) with extracted viral DNA.
Fig. 2 Real-time RT-PCR result(HCV) of viral RNA extracted from serum
Real-time RT-PCR results using an AccuPower® HCV Quantitative PCR Kit(HCV-1111, Bioneer) and run on Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060, Bioneer) with extracted viral RNA.
Specifications
Specifications
| Starting sample | variable |
| Elution volume | variable |
| Expected Yield | variable |
Content
| Components | Quantity | Note |
| Buffer Cartridge ① | 6 ea | |
| Buffer Cartridge ② | 6 ea | |
| Disposable filter tip | 3 pack | 32 ea/pack |
| Reaction tube | 1 pack | 96 ea/pack |
| Elution tube | 1 pack | 12 ea/pack |
| Manual | 1 ea |
Supporting Data
References
Resources
Manual
Manual
Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
• EN ISO 13485:2003 / AC:2009 - certificate
