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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

ExiProgen™ Tissue RNA Kit


  • Product from BIONEER

  • Reproducible results of automated extraction of RNA from various tissue samples

  • Excellent price-value ratio

  • High purity and high yield RNA with minimum hands-on time

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Description

ExiProgen™ Tissue total RNA Kit uses Tissue lysis buffer ① and Tissue lysis buffer ② to efficiently disrupt animal tissues and proteins for efficient extraction of total RNA without degradation. The binding buffer and silica magnetic particles will bind total RNA to the surface of the beads. The reaction containers are then subject to a magnetic field, forcing the total RNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers +are introduced sequentially during in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the total RNA-bound silica magnetic particles remain bound by the magnetic field. RNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified total RNA.

The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance. The buffer composition was developed to extract high purity and high yield NA suitable for the use in all  further applications.The extraction protocols programmed on the instrument were optimized for maximum performance. Everybody can extract NA of high purity and yield using Bioneer instruments and automated kits.

Procedure

 

Experimental Data

Fig. 1
Fig. 1 Electrophoresis result with LabChip GX
Results of capillary electrophoresis of total RNA extracted from tissue sample(rat liver, 15mg) using LabChip GX(Caliper life science, USA). Average RQS(RNA Quality Score) were at least 9.4.

 

Fig. 2
Fig. 2 Real-time RT-PCR results of total RNA extracted from animal tissue
Real-time RT-PCR results using an AccuPower® RocketScrpt™ RT PreMix(K-2101, Bioneer)와 AccuPower® Dualstar™ qPCR PreMix(K-6110, Bioneer) and run on ExicyclerTM 96 Real-Time Quantitative Thermal Block (A-2060, Bioneer) from total RNA extracted from liver sample (rat, 15mg).

Specifications

Specifications

  Liver Eye-ball Brain Tail-tip
Sample Vol. 15 mg 1 ea 20 mg 15 mg
Elution Vol. 100 ul 100 ul 100 ul 100 ul
Yield (ug) 25 ug 10 ug 8.5 ug 4 ug
Purity (A260/280) > 1.9 > 1.9 > 1.9 > 1.9

Content

Components Quantity Note
Buffer Cartridge ① 6 ea  
Buffer Cartridge ② 6 ea  
Tissue lysis buffer ① 1 ea 50 ml
Tissue lysis buffer ② 1 ea 50 ml
Disposable filter tip 3 pack 32 ea/pack
Elution tube 1 pack 12 ea/pack
Manual 1 ea  

Supporting Data

 Manual

ExiProgen

 Brochure

References

Resources

 Manual

ExiProgen

 Brochure

ExiProgen™ - 2013 Brochure

 Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

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