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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

ExiProgen™ Blood Genomic DNA Kit


  • Product from BIONEER

  • Reproducible results of automated extraction of genomic DNA from blood

  • Excellent price-value ratio

  • High purity and high yield DNA with minimum hands-on time

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Description

ExiProgen™ Blood Genomic DNA Kit is designed for automated DNA extraction from blood using ExiProgen™ instrument.

Kit uses a unique Tissue lysis buffer with Proteinase K to lyse eliminate proteins for efficient extraction of DNA. The binding buffer and silica magnetic particles will bind DNA to the surface of the beads. The wells are then subject to a magnetic field, forcing the DNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially in the next step to remove the remainder of cellular waste products. The washing buffers are then pipetted off, while the DNA-bound silica magnetic particles remain bound by the magnetic field. DNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified DNA.

The product is produced under ISO 9001 quality standards by an automated production facility to provide you with high-quality performance. The buffer composition was developed to extract high purity and high yield NA suitable for the use in all  further applications.The extraction protocols programmed on the instrument were optimized for maximum performance. Everybody can extract NA of high purity and yield using Bioneer instruments and automated kits

Procedure




Experimental Data



Fig. 1 Agarose gel electrophoresis results of genomic DNA extracted from bovine tissue
Results of gel electrophoresis of genomic DNA extracted from 40mg bovine tissue sample. Yields were on average 10ug and purities (A260/A280) were at least (average) 1.8. Also, to observe cross-contamination that may occur during the extraction process, the mouse tissue samples were placed in a checkerboard pattern, and the rest of the wells were filled with ddH2O. No cross-contamination could be detected.
M; Size marker, S; Extraction with bovine tissue sample, B; Extraction with D.W only.

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Specifications

Specifications

   Starting sample up to 200 ul
   Elution volume 50 - 100 ul
   Expected Yield 5 - 10 ug
   Expected Purity (A260/280) > 1.8

Content

Components Quantity Note
Buffer Cartridge ① 6 ea  
Buffer Cartridge ② 6 ea  
Tissue lysis buffer 1 ea 25 ml
Disposable filter tip 3 pack 32 ea/pack
Elution tube 1 pack 12 ea/pack
Manual 1 ea  
 

Supporting Data

 Manual

ExiProgen

 Brochure

References

Resources

 Manual

ExiProgen

 Brochure

ExiProgen™ - 2013 Brochure

 Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

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