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EXCELLENT TOOLS & SERVICES FOR DNA, RNA, PROTEIN OR CELL RESEARCH

CycleScript™ Reverse Transcriptase


  • Product from BIONEER

  • CycleScriptTM Reverse Transcriptase - engineered enzyme for high yields of cDNA

  • Temperature range (42°C –55°C) allows efficient cDNA synthesis from complex RNA

  • Versatile enzyme - 9 kb in 10 minutes when used under Cycling RT conditions (patent pending)

  • Economical package - in addition to the buffer, includes dNTPs

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Description

Get more cDNA in less time with CycleScript Reverse Transcriptase. CycleScript is a versatile reverse transcriptase - applicable to both conventional Reverse Transcription and Cyclic Reverse Transcription (Cyclic RT, patent pending – for cDNA amplification). It features high activity across a wide range of temperatures from 37 to 55°C therefore, reverse transcription is carried out like PCR. The Cyclic RT reaction has following steps: 1st incubation at 15~40°C for primer annealing, heating up to 42~48°C for extension, and finally incubation at 50~55°C for denaturation of the secondary structure of the RNA (optional). Bioneer's novel Cyclic RT system offers homogeneous cDNA synthesis, with a high yield of cDNA up to 9 kb.

Specifications

Enzyme Properties

  • 5' to 3' exonuclease activity: No
  • 3' to 5' exonuclease activity: No
  • 3’ – A Overhang: Yes
  • Strand Displacement: Yes
  • Fragment Size: Up to 9 kb

Applications

  • First-strand synthesis of cDNA from RNA molecules
  • RT-PCR
  • Random priming reaction
  • Library construction
  • Probe labeling
  • mRNA 5’ end mapping by primer extension analysis

Reagents Supplied

  • 5 x Reaction Buffer: 50 mM Tris-HCl, 250 mM KCl, 10 mM MgCl2, pH 8.1
  • 100 mM DTT
  • dNTP Mix: 2.5 mM of each dNTP

Concentration

200 U/μl

Storage Conditions

50% glycerol containing 20 mM Tris-Cl (pH7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % IGEPAL CA-630

Store Temperature

-20°C

Unit Definition

One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly (A)ooligo (dT) as template primer.

Supporting Data

References

Resources

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