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AccuPower® DualStar qPCR PreMix


  • Product from BIONEER

  • Lyophilized qPCR PreMix for highly specific probe-based qPCR, supplied with water for mix dilution

  • High sensitivity, great specificity, low background

  • Mastermix versions optimized for different instruments, mastermix for ABI 7500 provided with separate tube of ROX

  • Lyophilized mastermix is stable at room temperature for a month and for 2 years at -20°C

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Description

AccuPower® PreMix series are an innovative PCR mastermixes with a wide range of applications and unparalleled ease of use and stability. Mastermixes include all reaction components and are prealiquoted into tubes or plates and lyophilized with a patented stabilizer that maintains full activity for over one month at room temperature, and two years in the freezer. In addition, PCR enzyme stability is maintained at 95°C for up to 3 times longer than Taq DNA polymerase alone, making our mastermixes an ideal choice for better yields and G:C rich templates.

The AccuPower® DualStar™ qPCR PreMix contains all reaction components for highly specific probe-based qPCR in prealiquoted lyophilized format and provides high sensitivity, low background amplification. Unique hot start enzyme in combination with patented stabilizer allows for a greater specificity and more robust performance. Just add template, primers and labeled probe  into DualStar™ qPCR PreMix and enjoy good and reproducible results. Lyophilized mastermix is stable at room temperature for a month and for 2 years at -20oC, it is provided with water for resolving the lyophilizate.

AccuPower® DualStar™ qPCR PreMix is available in different versions, each optimized for a certain qPCR cycler. For instruments where ROX passive reference dye is required (like ABI 7500), the mix is supplied with a separate ROX vial.




Specifications

Specifications

  • 5' to 3' exonuclease: Yes
  • 3' to 5' exonuclease: No
  • 3’ – A Overhang: Yes
  • Fragment size: ~1 kb

Applications

  • Real-Time quantification of DNA and cDNA targets
  • Gene expression profiling
  • Microbial & viral pathogen detection
  • Evaluation of probe-based real-time PCR system

Supporting Data

References

Resources

Manual

AccuPower® DualStar™ qPCR PreMix

MSDS

• MSDS_AccuPower® DualStar™ qPCR PreMix

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485:2003 / AC:2009 - certificate

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